Ility of OFFGELTM to properly and reproducibly individual milligram qu…
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Ility of OFFGELTM to correctly and reproducibly independent milligram portions of protein into 12 fractionsO'Cualain et al. Malaria Journal 2010, nine:286 http://www.malariajournal.com/content/9/1/Page 12 of11. Ernoult E, Bourreau A, Gamelin E, Guette C: A proteomic strategy for plasma biomarker discovery with iTRAQ labelling and OFFGEL fractionation. J Biomed Biotechnol 2010, 2010:927917. twelve. Mulvenna J, Hamilton B, Nagaraj SH, Smyth D, PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/11011031 Loukas A, Gorman JJ: Proteomics Analysis from the Excretory/Secretory Ingredient from the Bloodfeeding Phase from the Hookworm, Ancylostoma caninum. Mol Mobile Proteomics 2009, 8:109-121. 13. Nirmalan N, Sims PFG, Hyde JE: Quantitative proteomics with the human malaria parasite Plasmodium falciparum and its application to studies of progress and inhibition. Mol Microbiol 2004, 52:1187-1199. fourteen. Read through M, Hyde JE: Easy in vitro cultivation of your malaria parasite Plasmodium falciparum (Erythrocytic phases) appropriate for large-scale preparations. Procedures Carbonic Anhydrase 1, Human (His) Mol Biol; Protocols in molecular parasitology 1993, 21:43-55. fifteen. Walliker D, Beale G: Synchronization and cloning of malaria parasites. Strategies Mol Biol; Protocols in molecular parasitology 1993, 21:57-66. 16. Laemmli Uk: Cleavage of Structural Proteins During Assembly of Head of Bacteriophage-T4. Nature 1970, 227:680-685. 17. Rabilloud T: Solubilization of proteins for electrophoretic analyses. Electrophoresis 1996, 17:813-829. eighteen. Hubner NC, Ren S, Mann M: Peptide separation with immobilized pI strips is really an desirable option to in-gel protein digestion for proteome examination. Proteomics 2008, eight:4862-4872. 19. Apweiler R, Martin MJ, O'Donovan C, Magrane M, Alam-Faruque Y, Antunes R, Barrell D, Bely B, Bingley M, Binns D, Bower L, Browne P, Chan WM, Dimmer E, Eberhardt R, Fedotov A, Foulger R, Garavelli J, Huntley R, Jacobsen J, Kleen M, Laiho K, Leinonen R, Legge D, Lin Q, Liu WD, Luo J, Orchard S, Patient S, Poggioli D, Pruess M, Corbett M, di Martino G, Donnelly M, van Rensburg P, Bairoch A, Bougueleret L, Xenarios I, Altairac S, Auchincloss A, Argoud-Puy G, Axelsen K, Baratin D, Blatter MC, Boeckmann B, Bolleman J, Bollondi L, Boutet E, Quintaje SB, Breuza L, Bridge A, deCastro E, Ciapina L, Coral D, Coudert E, Cusin I, Delbard G, Doche M, Dornevil D, Roggli PD, Duvaud S, Estreicher A, Famiglietti L, Feuermann M, Gehant S, Farriol-Mathis N, Ferro S, Gasteiger E, Gateau A, Gerritsen V, Gos A, Gruaz-Gumowski N, Hinz U, Hulo C, Hulo N, James J, Jimenez S, Jungo F, Kappler T, Keller G, Lachaize C, LaneGuermonprez L, Langendijk-Genevaux P, Lara V, Lemercier P, Lieberherr D, Lima TD, Mangold V, Martin X, Masson P, Moinat M, Morgat A, Mottaz A, Paesano S, Pedruzzi I, Pilbout S, Pillet V, Poux S, Pozzato M, Redaschi N, Rivoire C, Roechert B, Schneider M, Sigrist C, Sonesson K, Staehli S, Stanley E, Stutz A, Sundaram S, Tognolli M, Verbregue L, Veuthey AL, Yip LN, Zuletta L, Wu C, Arighi C, Arminski L, Barker W, Chen CM, Chen YX, Hu ZZ, Huang HZ, Mazumder R, McGarvey P, Natale DA, Nchoutmboube J, Petrova N, Subramanian N, Suzek BE, Ugochukwu U, Vasudevan S, Vinayaka CR, Yeh LS, Zhang J, UniProt C: The Universal Protein Resource (UniProt) in 2010. Nucleic Acids Res 2010, 38:D142-D148. 20. Malaria parasite metabolic pathways:[http://sites.huji.ac.il/malaria/]. 21. Foth BJ, Zhang N, Mok S, Preiser PR, Bozdech Z: Quantitative protein expression profiling reveals intensive post-transcriptional regulation and post-translational modifications in schizont-stage malaria par.
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